منابع مشابه
Structure of bacterial luciferase.
The generation of light by living organisms such as fireflies, glow-worms, mushrooms, fish, or bacteria growing on decaying materials has been a subject of fascination throughout the ages, partly because it occurs without the need for high temperatures. The chemistry behind the numerous bioluminescent systems is quite varied, and the enzymes that catalyze the reactions, the luciferases, are a l...
متن کاملA cyanide-aldehyde complex inhibits bacterial luciferase.
Cyanide at high (millimolar) concentrations inhibited in the in vitro Vibrio harveyi luciferase reaction. Cyanide reacted with free aldehyde to form an inhibitor. Inhibitor formation was accelerated by alkaline conditions and bovine serum albumin.
متن کاملCrystalline Bacterial Luciferase from Photoberterium Fischeri.
13 Yphantis, D. A., Biochemistry, 3, 297 (1964). We are indebted to Dr. Vern Schumaker for the determination of the molecular weights by this method. 14 Arnaud, C., C. Anast, and A. Tenenhouse, in preparation. 15 Rasmussen, H., C. Arnaud, and C. Hawker, Science, 144, 1019 (1964). 16 Moore, S., personal communication. We are indebted to Dr. Moore for several helpful discussions during the course...
متن کاملIsolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction
Procedures and conditions have been established such that the unstable enzyme-bound flavin intermediate produced in the bacterial luciferase reacti~ can be isolated as approximately 70% of the flavin product, the remaining being the final product, FMN. The structure of the intermediate is proposed to be that of a luciferase-bound 4a,5-dihydroflavin-4a-hydroxide. The intermediate has a half-life...
متن کاملInteraction of bacterial luciferase with 8-substituted flavin mononucleotide derivatives.
Bacterial luciferase catalyzes the emission of visible light from the reaction of reduced flavin, molecular oxygen, and an n-alkyl aldehyde. The mechanism of the reaction was probed by measuring the electronic effects of various substituents at the 8-position of the flavin ring system. Substituent effects were obtained for CH3-, Cl-, CH3O-, CH3S-, F-, and H- on the rate of formation and decay o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42742-7